Why Is This Important?
Utilizing algae in food, nutraceutical, or cosmetic products requires comprehensive testing to harness its benefits effectively while ensuring safety and compliance. Given the natural variability in algae, understanding these variations is critical for successful product development.
Key Testing Considerations
Health and safety
Health and safety testing ensures the algae-based product is safe for human consumption or topical application by detecting potential contaminants and can be reviewed against the regulatory requirements for its intended use.
Nutritional
Nutritional tests provide the information needed for an FDA compliant nutritional label and provide an overview of the algae’s nutritional content: calories, carbohydrates, fats, proteins, and common mineral content.
Bioactivity testing
One of the things that makes algae unique is its bioactive properties. These are specific polysaccharides (carbohydrates), amino acids, or mineral content that provide unique value to the body.
If you’re thinking of algae or algae-based extracts in a product, you probably want to speak to these properties to help a customer understand why they should choose your product over an alternative and command a higher price in the market.
Rather than just measuring the overall carbohydrate or protein content, you’ll want to drill down into the specific polysaccharides or amino acids in your algae and their concentration so you can use them to provide higher value benefits.
Measures arsenic, cadmium, lead, and mercury levels utilizing mass spectrometry.
Samples are digested with HNO3 in an open- or closed- vessel microwave digestion system. Analysis is performed using an inductively coupled plasma with mass spectrometric detection (ICP-MS). The digested samples are compared to standards of known concentration. LOQ varies depending on matrix.
Arsenic 110 ppb, Cadmium 15 ppb, Lead 15 ppb, Mercury 15 ppb.
AOAC 2011.19, 993.14 and 2015.01 (modified)
10 grams in dry format
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Tests for various forms of microorganisms that are commonly tested for products being used in food, nutraceutical, and cosmetic applications.
Measures the number of aerobic bacteria present in a sample. It doesn't specify bacteria types but gives an overall indication of the microbial population.
Test for this specific type of bacteria, often found on the skin and in the respiratory tract of humans.
Test for a broad class of bacteria found in the environment, including the feces of animals and humans. While most strains are harmless, some can cause serious food poisoning.
Test for microorganisms that can cause food spoilage. Some species can produce toxins harmful to humans or animals.
Measures for a type of bacteria that can cause foodborne illness. Found in a variety of contaminated foods.
150 grams in dry format
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Series of tests and calculations that cover the FDA's nutrition and labeling requirements for food and dietary supplements. Includes testing for: ash, calcium, calories, cholesterol in food, fatty acid composition, fiber, iron, moisture, potassium, protein, sodium, sugar profile, vitamin D.
This analysis includes the following tests:
Below is a description and methods of the individual tests:
2-5 grams of sample is weighed into crucible and placed in a 550-600°C furnace. The sample is ashed in the furnace for a minimum of 4 hours or until fully ashed and no glowing embers remain. Sample is cooled and weightof the ash is determined.
Sample is digested using an appropriate method, typically dry ashing, though wet ashing isused for predominantly inorganic samples. The resultant digest is analyzed by Inductively Coupled Plasma OpticalEmission Spectrophotometry against a set of ISO certified standards.
Atwater calculation: 4 x g (carbs) + 9 x g (fat) + 4 x g (protein).
Atwater formula: 9 x g(fat).
Carbohydrates = 100 - (protein + fat + Moisture + ash).
Sample is saponified with potassium hydroxide. A liquid-liquid extraction is performed, collecting the unsaponifiable fraction in toluene. An aliquot of the toluene extract is dried down, and then redissolved in dimethylformamide. The sterols in the concentrated extract are derivatized to trimethylsilyl ethers which are then extracted into heptane containing internal standard. The heptane is injected into a gas chromatograph, and the cholesterol determined from a standard curve of known standards and an internal standard.
An appropriate fat extraction is performed on samples that are not pure fats. Sample or extracted fat from sample is reacted with boron-trifluoride/methanol reagent to convert fatty acids present in any form into their methyl ester forms. These are then extracted into hexane, and injected onto a capillary column gas chromatograph. Standards of known composition are used to identify the fatty acids present and the percentage of each fatty acid as a part of the entire sample calculated.
Where applicable, fat and or sugar are extracted, dried down samples undergo enzymatic digestion to remove starch and protein, obtaining dietary fiber.
Sample is digested using an appropriate method, typically dry ashing, though wet ashing is used for predominantly inorganic samples. The resultant digest is analyzed by Inductively Coupled Plasma Optical Emission Spectrophotometry against a set of ISO certified standards.
Sample is weighed into a dish and placed into a vacuum oven for a specific amount of time depending on sample matrix. This time is at least 5 hours. Depending on the sample matrix and the sugar content, the sample can be dried at a temperature of approximately 100°C. 60°C is used for samples containing high amounts of sugars.
The resultant digest is analyzed by Inductively Coupled Plasma Optical Emission Spectrophotometry against a set of ISO certified standards.
Sample is entered into the combustion chamber of a protein analyzer, in which the gas from the combustion is analyzed for nitrogen content and calculated to protein.
Sample is digested using an appropriate method, typically dry ashing, though wet ashing is used for predominantly inorganic samples. The resultant digest is analyzed by Inductively Coupled Plasma Optical Emission Spectrophotometry against a set of ISO certified standards.
The sample is extracted with alcohol/water. The extract is prepped for chromatography and separated on an amino propyl HPLC column with an ELSD- Evaporative Light Scattering Detector.
Samples are saponified and all fat-soluble compounds are extracted into hexane solution. Deuterated Vitamin D2 and D3 is used as internal standards. This hexane phase, now containing the vitamin D, is evaporated to dryness, reconstituted in a mixture of acetonitrile and water. Separations are performed on UPLC and quantified on a MS/MS detector.
Available upon request
575 grams in dry format
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Measures erythritol, isomalt, lactitol, maltitol, mannitol, sorbitol, xylitol using high- performance anion exchange chromatography (HPAEC).
Sugar alcohols are extracted with water and a chloroform extraction is performed to remove lipids and denature protein. After centrifugation, the sample is injected onto a high- performance anion exchange chromatography (HPAEC) system equipped with a pulsed amperometric detector (PAD). This test measures:
20 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Measures fructose, glucose, sucrose, maltose, and lactose using HPLC chromatography.
The sample is extracted with alcohol/water. The extract is prepped for chromatography and separated on an amino propyl HPLC column with an ELSD- Evaporative Light Scattering Detector. This test measures the following:
AOAC 982.14, mod.
50 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Measures 17 different amino acids using high-performance liquid chromatography (HPLC).
The samples are hydrolyzed in 6 N Hydrochloric acid for 24 hours at approximately 110 degrees C. Phenol is added to the 6N Hydrochloric acid to prevent halogenation of tyrosine. Cystine and Cysteine are converted to S-2-carboxyethylthiocysteine by the addition of dithiodipropionic acid. The samples are analyzed by HPLC after pre-injection derivitization. The primary amino acids are derivitized with o-phthalaldehyde (OPA) and the secondary amino acids are derivitized with fluorenylmethyl chloroformate (FMOC) before injection.
Note: Tryptophan is Not measured by this method, and requires a separate analysis with base hydrolysis (TRPLC_S / FS05E).
Agilent Application Note 5990-4547, (2010); J of Chromatography, 431, pp 271- 284; Analytical Biochemistry 177, 318-322
5 grams in dry, milled form
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Measures total polyphenols spectrophotometrically.
Polyphenols are extracted from the sample. The sample is reacted with Folic-Ciocalteu Reagent (FCR) at an alkaline pH to produce a chromophore that can be measured spectrophotometrically at 760 nm. Results are reported in units of mg/g gallic acid equivalents.
Total Polyphenols: 12 mg/g.
Methods in Enzymology 1999, Vol. 299, Modified
20 grams in dry, milled form
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Uronic acids content is quantified using colorimetry.
All polysaccharides are hydrolyzed to monosaccharides by acidic hydrolysis. The uronic acid content is quantified, after specific treatment, by colorimetry.
250 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Sugars are extracted by refluxing the sample with dilute sulfuric acid. After the extract is neutralized, an aliquot is dried under inert gas. The sugars are converted to oximes, which are then converted to aldonitrile peracetates and analyzed by gas chromatography (GC) using a flame ionization detector. The test measures:
Analytical Chemistry, Volume 38(2) (modified)
6 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Sugars are extracted by refluxing the sample with dilute sulfuric acid. After the extract is neutralized, an aliquot is dried under inert gas. The sugars are converted to oximes, which are then converted to aldonitrile peracetates and analyzed by gas chromatography (GC) using a flame ionization detector. The test measures:
Analytical Chemistry, Volume 38(2) (modified)
6 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
This analysis includes the following tests:
Samples are either dry ashed, wet ashed, or read directly. If dry ashed, the sample is placed in a muffle furnace set to maintain 500 deg C until ashing is complete. The resulting ash is treated with concentrated hydrochloric acid, dried and redissolved in hydrochloric acid solution. If wet ashed, the sample is digested in a microwave or on a hot plate with nitric acid, hydrochloric acid, and/or hydrogen peroxide. The amount of each element is determined with an ICP spectrometer by comparing the emission of the unknown sample against the emission of each element from standard solutions.
Sample is digested with a mixture of acids. This process breaks down the organic matter, releasing iodine and other elements into the solution. The digested sample is introduced into in an Inductively Coupled Plasma (ICP) specrometer. This instrument uses a high-temperature plasma to excite the atoms in the sample. When these atoms return to their ground state, they emit light at specific wavelengths. The ICP spectrometer is capable of detecting the specific wavelength of light emitted by iodine atoms. By measuring the intensity of this light, the concentration of iodine in the seaweed sample can be quantified. The results are usually expressed in mg/kg (milligrams of iodine per kilogram of seaweed). These results can then be used to assess the iodine content in the seaweed, ensuring it meets nutritional standards or requirements.
Sample is digested by microwave under pressure in concentrated acid. Sample digest is then introduced to the ICP-MS. The selenium signal in the sample is compared to a set of NIST traceable standards to determine the concentration.
130 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
This analysis includes the following tests:
Samples are either dry ashed, wet ashed, or read directly. If dry ashed, the sample is placed in a muffle furnace set to maintain 500 deg C until ashing is complete. The resulting ash is treated with concentrated hydrochloric acid, dried and redissolved in hydrochloric acid solution. If wet ashed, the sample is digested in a microwave or on a hot plate with nitric acid, hydrochloric acid, and/or hydrogen peroxide. The amount of each element is determined with an ICP spectrometer by comparing the emission of the unknown sample against the emission of each element from standard solutions.
Sample is digested with a mixture of acids. This process breaks down the organic matter, releasing iodine and other elements into the solution. The digested sample is introduced into in an Inductively Coupled Plasma (ICP) specrometer. This instrument uses a high-temperature plasma to excite the atoms in the sample. When these atoms return to their ground state, they emit light at specific wavelengths. The ICP spectrometer is capable of detecting the specific wavelength of light emitted by iodine atoms. By measuring the intensity of this light, the concentration of iodine in the seaweed sample can be quantified. The results are usually expressed in mg/kg (milligrams of iodine per kilogram of seaweed). These results can then be used to assess the iodine content in the seaweed, ensuring it meets nutritional standards or requirements.
Sample is digested by microwave under pressure in concentrated acid. Sample digest is then introduced to the ICP-MS. The selenium signal in the sample is compared to a set of NIST traceable standards to determine the concentration.
130 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
This add-on measures the complete vitamin profile. It measures the following vitamins:
Below is a description and methods of the individual tests conducted as part of the analysis:
Determination of Vitamin A is used to assay food, wet and dry pet food, feed, premixes, and dietary supplements for their retinol, α-carotene, and β-carotene content. Total vitamin A is equivalent to the sum of retinol and β-carotene concentrations. In this method, samples are weighed into saponification flasks. Samples are saponified on a steam bath with alcohol, potassium hydroxide, and an antioxidant. Samples are then cooled, hexane is added, and samples are mixed. Phases are allowed to separate. The organic layer containing the vitamin A is decanted into a separatory funnel. This extraction process is performed a total of three times for most sample matrices and at least five times for high level samples to ensure complete extraction. The organic collection in the funnels is rinsed with DI water and finally filtered through sodium sulfate into volumetric flasks.
Thiamine is extracted into 0.1 HCL in an autoclave. Phosphorylated thiamine derivatives are converted into free thiamine with the background phosphatase activity of an α-amylase solution. Analysis is performed on an ultra-performance liquid chromatograph with post column oxidation of thiamine to thiochrome and fluorescence detection.
Riboflavin is extracted into 0.1 HCL in an autoclave. Phosphorylated riboflavin derivatives are converted into free thiamine with the background phosphatase activity of an α-amylase solution. Analysis is performed on an ultra-performance liquid chromatograph with fluorescence detection.
This method is used for the microbiological analysis of total niacin/niacinamide in food, wet and dry pet food, feed, commodities, and dietary supplements. This method cannot distinguish different forms of niacin. Niacin (vitamin B3), which is in the B complex series of water-soluble vitamins, is important for human and animal nutrition. It is an essential component of the enzyme systems for the metabolism of carbohydrates, fatty acids, and amino acids. Niacin is widely distributed in foods and feed. As it occurs naturally, it is bound to other chemical compounds, thus it must be freed by hydrolysis with either strong acid or alkali. Samples are extracted by autoclaving for 30 minutes in solution containing distilled water and 1 N H2SO4. The pH of each sample is then adjusted to 6.8 using NaOH and H2SO4 solutions, followed by a series of dilutions as necessary based on the expected level of niacin in the sample.
Pantothenic Acid is a water-soluble B vitamin. It is required to synthesize coenzyme-A, as well as to synthesize and metabolize proteins, carbohydrates, and fats. It is found in nearly every food, with high amounts in whole-grain cereals, legumes, eggs, meat, avocado, and yogurt. It is also called Vitamin B5 or pantothenate. This method is a microbiological analysis. Samples are extracted by autoclaving for 5 minutes in acetate buffer. The pH of sample solutions is adjusted to 6.8, followed by serial dilutions as necessary. Sample solution is then mixed with growth media and inoculated with L. plantarum. After overnight incubation at 37oC, the concentration of pantothenic acid in the sample is determined by reading the turbidity of the sample.
This method can analyze total vitamin B6, which include pyridoxine, pyridoxal and pyridoxamine. The sample is incubated with phosphatase with shaking overnight at 37C to free bound vitamin B6. Pyridoxamine is converted into pyridoxal by reaction with glyoxylic acid in the presence of Fe2+ catalyst. Pyridoxal is then reduced to pyridoxine by the action of sodium borohydride in the alkaline medium. Total vitamin B6 is separated on a C8 column with fluorescence detection.
Folate is a general term that includes both folic acid (vitamin B9) and poly-γ-glutamyl conjugates exhibiting the biological activity of folic acid. MET3389 - Total Folate provides instructions for the microbiological analysis of folate in food, feed, wet and dry pet food, commodities, and nutritional supplements. In this method, samples are autoclaved and undergo enzymatic treatment using Creon capsules and chicken pancreas conjugase to release folate from the matrix. After overnight incubation, the concentration of total folate in the sample is determined by reading the turbidity of the sample against that of a series of calibration standards. The amount of growth is directly proportional to the concentration of the analyte in the sample.
Vitamin B12, also called cobalamin, is a water-soluble vitamin. It has a key role in the functioning of the brain and nervous system, as well as the formation of blood. Vitamin B12 can be found in fish, shellfish, meat, liver, poultry, eggs, milk, and milk products. A synthetic form of the vitamin, called Cyanocobalamin, is used in supplements and as a food additive. This method is a microbiological analysis. Samples are extracted by autoclaving for 10 minutes in extraction solution which contains sodium metabisulfite, sodium phosphate dibasic and citric acid. The pH of sample solution is adjusted, followed by serial dilutions as necessary. Sample solution is then mixed with growth media and inoculated with L. Delbrueckii. After overnight incubation at 37 °C, the concentration of B12 in the sample is determined by reading the turbidity of the sample. The amount of growth is directly proportional to the concentration of the analyte in the sample.
Samples are saponified and all fat-soluble compounds are extracted into hexane solution. Deuterated Vitamin D2 and D3 is used as internal standards. This hexane phase, now containing the vtiamin D, is evaporated to dryness, reconstituted in a mixture of acetonitrile and water. Separations are performed on UPLC and quantified on a MS/MS detector.
This method determines the amount of vitamin E in a sample by individually quantifying the levels of four tocopherol isomers: alpha (α), beta (β), gamma (γ), and delta (δ) and four tocotrienol. isomers: alpha (α), beta (β), gamma (γ), and delta (δ). The method can be used on most foods, feeds, commodities, and premixes formulated with mixed tocopherol isomers, and on nutritional supplements. To analyze for Vitamin E, the sample is weighed into a saponification flask and an antioxidant, alcohol, and potassium hydroxide are added to the flask. The flask is refluxed over a steam bath for 15 minutes. After cooling, vitamin E is extracted using a liquid/liquid extraction with a petroleum ether/ethyl acetate solution.
The sample is extracted with organic solvents and injected on reverse phase high-performance liquid chromatography (HPLC) system with post column
reduction and fluorescence detection.
550 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Sugars are extracted by refluxing the sample with dilute sulfuric acid. After the extract is neutralized, an aliquot is dried under inert gas. The sugars are converted to oximes, which are then converted to aldonitrile peracetates and analyzed by gas chromatography (GC) using a flame ionization detector. The test measures:
Analytical Chemistry, Volume 38(2) (modified)
6 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
When algae is not stored right after harvesting, microbes can form that create allergens and can be as dangerous as heavy metals. This test uses Ion Chromatography and measures the following items:
200 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Various arsenic species are extracted from samples in plastic vessels with dilute nitric acid using oven digestion and then filtered prior to analysis. Test is for marine life and marine samples.
ICP-MS, AAS
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
This add-on measures the complete vitamin profile. It measures the following vitamins:
Below is a description and methods of the individual tests conducted as part of the analysis:
Determination of Vitamin A is used to assay food, wet and dry pet food, feed, premixes, and dietary supplements for their retinol, α-carotene, and β-carotene content. Total vitamin A is equivalent to the sum of retinol and β-carotene concentrations. In this method, samples are weighed into saponification flasks. Samples are saponified on a steam bath with alcohol, potassium hydroxide, and an antioxidant. Samples are then cooled, hexane is added, and samples are mixed. Phases are allowed to separate. The organic layer containing the vitamin A is decanted into a separatory funnel. This extraction process is performed a total of three times for most sample matrices and at least five times for high level samples to ensure complete extraction. The organic collection in the funnels is rinsed with DI water and finally filtered through sodium sulfate into volumetric flasks.
Thiamine is extracted into 0.1 HCL in an autoclave. Phosphorylated thiamine derivatives are converted into free thiamine with the background phosphatase activity of an α-amylase solution. Analysis is performed on an ultra-performance liquid chromatograph with post column oxidation of thiamine to thiochrome and fluorescence detection.
Riboflavin is extracted into 0.1 HCL in an autoclave. Phosphorylated riboflavin derivatives are converted into free thiamine with the background phosphatase activity of an α-amylase solution. Analysis is performed on an ultra-performance liquid chromatograph with fluorescence detection.
This method is used for the microbiological analysis of total niacin/niacinamide in food, wet and dry pet food, feed, commodities, and dietary supplements. This method cannot distinguish different forms of niacin. Niacin (vitamin B3), which is in the B complex series of water-soluble vitamins, is important for human and animal nutrition. It is an essential component of the enzyme systems for the metabolism of carbohydrates, fatty acids, and amino acids. Niacin is widely distributed in foods and feed. As it occurs naturally, it is bound to other chemical compounds, thus it must be freed by hydrolysis with either strong acid or alkali. Samples are extracted by autoclaving for 30 minutes in solution containing distilled water and 1 N H2SO4. The pH of each sample is then adjusted to 6.8 using NaOH and H2SO4 solutions, followed by a series of dilutions as necessary based on the expected level of niacin in the sample.
Pantothenic Acid is a water-soluble B vitamin. It is required to synthesize coenzyme-A, as well as to synthesize and metabolize proteins, carbohydrates, and fats. It is found in nearly every food, with high amounts in whole-grain cereals, legumes, eggs, meat, avocado, and yogurt. It is also called Vitamin B5 or pantothenate. This method is a microbiological analysis. Samples are extracted by autoclaving for 5 minutes in acetate buffer. The pH of sample solutions is adjusted to 6.8, followed by serial dilutions as necessary. Sample solution is then mixed with growth media and inoculated with L. plantarum. After overnight incubation at 37oC, the concentration of pantothenic acid in the sample is determined by reading the turbidity of the sample.
This method can analyze total vitamin B6, which include pyridoxine, pyridoxal and pyridoxamine. The sample is incubated with phosphatase with shaking overnight at 37C to free bound vitamin B6. Pyridoxamine is converted into pyridoxal by reaction with glyoxylic acid in the presence of Fe2+ catalyst. Pyridoxal is then reduced to pyridoxine by the action of sodium borohydride in the alkaline medium. Total vitamin B6 is separated on a C8 column with fluorescence detection.
Folate is a general term that includes both folic acid (vitamin B9) and poly-γ-glutamyl conjugates exhibiting the biological activity of folic acid. MET3389 - Total Folate provides instructions for the microbiological analysis of folate in food, feed, wet and dry pet food, commodities, and nutritional supplements. In this method, samples are autoclaved and undergo enzymatic treatment using Creon capsules and chicken pancreas conjugase to release folate from the matrix. After overnight incubation, the concentration of total folate in the sample is determined by reading the turbidity of the sample against that of a series of calibration standards. The amount of growth is directly proportional to the concentration of the analyte in the sample.
Vitamin B12, also called cobalamin, is a water-soluble vitamin. It has a key role in the functioning of the brain and nervous system, as well as the formation of blood. Vitamin B12 can be found in fish, shellfish, meat, liver, poultry, eggs, milk, and milk products. A synthetic form of the vitamin, called Cyanocobalamin, is used in supplements and as a food additive. This method is a microbiological analysis. Samples are extracted by autoclaving for 10 minutes in extraction solution which contains sodium metabisulfite, sodium phosphate dibasic and citric acid. The pH of sample solution is adjusted, followed by serial dilutions as necessary. Sample solution is then mixed with growth media and inoculated with L. Delbrueckii. After overnight incubation at 37 °C, the concentration of B12 in the sample is determined by reading the turbidity of the sample. The amount of growth is directly proportional to the concentration of the analyte in the sample.
Samples are saponified and all fat-soluble compounds are extracted into hexane solution. Deuterated Vitamin D2 and D3 is used as internal standards. This hexane phase, now containing the vtiamin D, is evaporated to dryness, reconstituted in a mixture of acetonitrile and water. Separations are performed on UPLC and quantified on a MS/MS detector.
This method determines the amount of vitamin E in a sample by individually quantifying the levels of four tocopherol isomers: alpha (α), beta (β), gamma (γ), and delta (δ) and four tocotrienol. isomers: alpha (α), beta (β), gamma (γ), and delta (δ). The method can be used on most foods, feeds, commodities, and premixes formulated with mixed tocopherol isomers, and on nutritional supplements. To analyze for Vitamin E, the sample is weighed into a saponification flask and an antioxidant, alcohol, and potassium hydroxide are added to the flask. The flask is refluxed over a steam bath for 15 minutes. After cooling, vitamin E is extracted using a liquid/liquid extraction with a petroleum ether/ethyl acetate solution.
The sample is extracted with organic solvents and injected on reverse phase high-performance liquid chromatography (HPLC) system with post column
reduction and fluorescence detection.
550 grams in dry, milled form.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
Cultured Supply maintains a pricing database of common algae and seaweed species across geographic regions. Any transaction and pricing data shared with Cultured Supply will be anonymized and added to the database to create a more efficient pricing index. Any personal or company-level information shared with Cultured Supply will be held strictly confidential and will not be shared with third parties. When Cultured Supply receives transaction data, we will respond with how this pricing compares in relation to the market index so you can gain additional insights into the overall market. Cultured Supply’s goal is to help create more structure and price stability in the algae market so businesses can make more informed decisions. Feel free to contact us at with any questions at info@culturedsupply.com.
